Literature summary extracted from

Aggarwal, S.; Brennen, W.N.; Kole, T.P.; Schneider, E.; Topaloglu, O.; Yates, M.; Cotter, R.J.; Denmeade, S.R.
Fibroblast activation protein peptide substrates identified from human collagen I derived gelatin cleavage sites (2008), Biochemistry, 47, 1076-1086.

Cloned(Commentary)

EC Number	Cloned (Comment)	Organism
3.4.21.B28	expressed in Schneider S2 cells	Homo sapiens

KM Value [mM]

EC Number	KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
3.4.21.B28	0.0202	-	7-methoxycoumarin-4-acetic acid-DSGETGPA-dinitrophenyl	in 100 mM NaCl, 100 mM Tris, pH 7.8, at 37°C	Homo sapiens
3.4.21.B28	0.021	-	7-methoxycoumarin-4-acetic acid-DRGETGPA-dinitrophenyl	in 100 mM NaCl, 100 mM Tris, pH 7.8, at 37°C	Homo sapiens
3.4.21.B28	0.0238	-	7-methoxycoumarin-4-acetic acid-DRGETGP-dinitrophenyl	in 100 mM NaCl, 100 mM Tris, pH 7.8, at 37°C	Homo sapiens
3.4.21.B28	0.0269	-	7-methoxycoumarin-4-acetic acid-EPGPPGPA-dinitrophenyl	in 100 mM NaCl, 100 mM Tris, pH 7.8, at 37°C	Homo sapiens
3.4.21.B28	0.0361	-	7-methoxycoumarin-4-acetic acid-ASGPAGPA-dinitrophenyl	in 100 mM NaCl, 100 mM Tris, pH 7.8, at 37°C	Homo sapiens
3.4.21.B28	0.0371	-	7-methoxycoumarin-4-acetic acid-ERGETGPAGG-dinitrophenyl	in 100 mM NaCl, 100 mM Tris, pH 7.8, at 37°C	Homo sapiens
3.4.21.B28	0.0396	-	7-methoxycoumarin-4-acetic acid-DSGETGP-dinitrophenyl	in 100 mM NaCl, 100 mM Tris, pH 7.8, at 37°C	Homo sapiens
3.4.21.B28	0.043	-	7-methoxycoumarin-4-acetic acid-ERGETGPAG-dinitrophenyl	in 100 mM NaCl, 100 mM Tris, pH 7.8, at 37°C	Homo sapiens
3.4.21.B28	0.046	-	7-methoxycoumarin-4-acetic acid-DKGESGPA-dinitrophenyl	in 100 mM NaCl, 100 mM Tris, pH 7.8, at 37°C	Homo sapiens
3.4.21.B28	0.051	-	7-methoxycoumarin-4-acetic acid-APGSKGDA-dinitrophenyl	in 100 mM NaCl, 100 mM Tris, pH 7.8, at 37°C	Homo sapiens
3.4.21.B28	0.0513	-	7-methoxycoumarin-4-acetic acid-ERGETGPSG-dinitrophenyl	in 100 mM NaCl, 100 mM Tris, pH 7.8, at 37°C	Homo sapiens
3.4.21.B28	0.052	-	7-methoxycoumarin-4-acetic acid-VGPAGK-dinitrophenyl	in 100 mM NaCl, 100 mM Tris, pH 7.8, at 37°C	Homo sapiens
3.4.21.B28	0.0567	-	7-methoxycoumarin-4-acetic acid-ERGETGPA-dinitrophenyl	in 100 mM NaCl, 100 mM Tris, pH 7.8, at 37°C	Homo sapiens

Localization

EC Number	Localization	Comment	Organism	GeneOntology No.	Textmining
3.4.21.B28	membrane	-	Homo sapiens	16020	-

Natural Substrates/ Products (Substrates)

EC Number	Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
3.4.21.B28	Gelatin + H2O	Homo sapiens	gelatin cleavage results in 51 fragments	?	-	?

Organism

EC Number	Organism	UniProt	Comment	Textmining
3.4.21.B28	Homo sapiens	-	-	-

Purification (Commentary)

EC Number	Purification (Comment)	Organism
3.4.21.B28	Ni-NTA resin column chromatography	Homo sapiens

Source Tissue

EC Number	Source Tissue	Comment	Organism	Textmining
3.4.21.B28	fibroblast	-	Homo sapiens	-

Substrates and Products (Substrate)

EC Number	Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
3.4.21.B28	7-methoxycoumarin-4-acetic acid-APGSKGDA-dinitrophenyl + H2O	-	Homo sapiens	?	-	?
3.4.21.B28	7-methoxycoumarin-4-acetic acid-ASGPAGPA-dinitrophenyl + H2O	-	Homo sapiens	?	-	?
3.4.21.B28	7-methoxycoumarin-4-acetic acid-DKGESGPA-dinitrophenyl + H2O	-	Homo sapiens	?	-	?
3.4.21.B28	7-methoxycoumarin-4-acetic acid-DRGETGP-dinitrophenyl + H2O	-	Homo sapiens	?	-	?
3.4.21.B28	7-methoxycoumarin-4-acetic acid-DRGETGPA-dinitrophenyl + H2O	-	Homo sapiens	?	-	?
3.4.21.B28	7-methoxycoumarin-4-acetic acid-DSGETGP-dinitrophenyl + H2O	-	Homo sapiens	?	-	?
3.4.21.B28	7-methoxycoumarin-4-acetic acid-DSGETGPA-dinitrophenyl + H2O	-	Homo sapiens	?	-	?
3.4.21.B28	7-methoxycoumarin-4-acetic acid-EPGPPGPA-dinitrophenyl + H2O	-	Homo sapiens	?	-	?
3.4.21.B28	7-methoxycoumarin-4-acetic acid-ERGETGPA-dinitrophenyl + H2O	-	Homo sapiens	?	-	?
3.4.21.B28	7-methoxycoumarin-4-acetic acid-ERGETGPAG-dinitrophenyl + H2O	-	Homo sapiens	?	-	?
3.4.21.B28	7-methoxycoumarin-4-acetic acid-ERGETGPAGG-dinitrophenyl + H2O	-	Homo sapiens	?	-	?
3.4.21.B28	7-methoxycoumarin-4-acetic acid-ERGETGPSG-dinitrophenyl + H2O	-	Homo sapiens	?	-	?
3.4.21.B28	7-methoxycoumarin-4-acetic acid-VGPAGK-dinitrophenyl + H2O	-	Homo sapiens	?	-	?
3.4.21.B28	Ala-Pro-7-amido-4-trifluoromethylcoumarin + H2O	-	Homo sapiens	Ala-Pro + 7-amino-4-trifluoromethylcoumarin + H2O	-	?
3.4.21.B28	denatured type I collagen + H2O	-	Homo sapiens	?	-	?
3.4.21.B28	Gelatin + H2O	gelatin cleavage results in 51 fragments	Homo sapiens	?	-	?

Synonyms

EC Number	Synonyms	Comment	Organism
3.4.21.B28	FAP	-	Homo sapiens
3.4.21.B28	fibroblast activation protein-alpha	-	Homo sapiens

Turnover Number [1/s]

EC Number	Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
3.4.21.B28	0.003	-	7-methoxycoumarin-4-acetic acid-DRGETGP-dinitrophenyl	in 100 mM NaCl, 100 mM Tris, pH 7.8, at 37°C	Homo sapiens
3.4.21.B28	0.003	-	7-methoxycoumarin-4-acetic acid-DSGETGP-dinitrophenyl	in 100 mM NaCl, 100 mM Tris, pH 7.8, at 37°C	Homo sapiens
3.4.21.B28	0.015	-	7-methoxycoumarin-4-acetic acid-APGSKGDA-dinitrophenyl	in 100 mM NaCl, 100 mM Tris, pH 7.8, at 37°C	Homo sapiens
3.4.21.B28	0.059	-	7-methoxycoumarin-4-acetic acid-DSGETGPA-dinitrophenyl	in 100 mM NaCl, 100 mM Tris, pH 7.8, at 37°C	Homo sapiens
3.4.21.B28	0.067	-	7-methoxycoumarin-4-acetic acid-DKGESGPA-dinitrophenyl	in 100 mM NaCl, 100 mM Tris, pH 7.8, at 37°C	Homo sapiens
3.4.21.B28	0.079	-	7-methoxycoumarin-4-acetic acid-ERGETGPAGG-dinitrophenyl	in 100 mM NaCl, 100 mM Tris, pH 7.8, at 37°C	Homo sapiens
3.4.21.B28	0.081	-	7-methoxycoumarin-4-acetic acid-DRGETGPA-dinitrophenyl	in 100 mM NaCl, 100 mM Tris, pH 7.8, at 37°C	Homo sapiens
3.4.21.B28	0.095	-	7-methoxycoumarin-4-acetic acid-VGPAGK-dinitrophenyl	in 100 mM NaCl, 100 mM Tris, pH 7.8, at 37°C	Homo sapiens
3.4.21.B28	0.115	-	7-methoxycoumarin-4-acetic acid-EPGPPGPA-dinitrophenyl	in 100 mM NaCl, 100 mM Tris, pH 7.8, at 37°C	Homo sapiens
3.4.21.B28	0.121	-	7-methoxycoumarin-4-acetic acid-ERGETGPAG-dinitrophenyl	in 100 mM NaCl, 100 mM Tris, pH 7.8, at 37°C	Homo sapiens
3.4.21.B28	0.141	-	7-methoxycoumarin-4-acetic acid-ERGETGPA-dinitrophenyl	in 100 mM NaCl, 100 mM Tris, pH 7.8, at 37°C	Homo sapiens
3.4.21.B28	0.196	-	7-methoxycoumarin-4-acetic acid-ASGPAGPA-dinitrophenyl	in 100 mM NaCl, 100 mM Tris, pH 7.8, at 37°C	Homo sapiens
3.4.21.B28	0.268	-	7-methoxycoumarin-4-acetic acid-ERGETGPSG-dinitrophenyl	in 100 mM NaCl, 100 mM Tris, pH 7.8, at 37°C	Homo sapiens

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Release 2023.1 (January 2023)